rabbit anti kcnq2  (Bioss)

Journal: Scientific Reports

Article Title: Multiple Transcriptomic Analyses Explore Potential Synaptic Biomarker Rabphilin-3A for Alzheimer's Disease

doi: 10.1038/s41598-024-66693-8

Figure Lengend Snippet: Gene expression patterns and differential analysis in neuron cells across Braak stages and disease states. ( A , C , E ) Violin plots depicting the significant differences in gene expression levels of KCNQ2, RPH3A, SV2A and TMEM59L between Braak stages 0 and 2, Braak stages 0 and 6, and Braak stages 2 and 6 (entorhinal cortex and superior frontal gyrus) as well as between AD and control samples (hippocampus) among excitatory and inhibitory neurons. *p < 0.05, **p < 0.01, ****p < 0.0001. ( B , D , F ) Corresponding gene expression patterns of 4 selected genes plotted on the UMAP coordinates in entorhinal cortex, superior frontal gyrus and hippocampus, respectively. These genes exhibit cell-type specificity in excitatory and inhibitory neurons. The color intensity corresponds to the expression level, with a gradient scale ranging from low (blue) to high (red) expression.

Article Snippet: After blocking and washing, the membranes were probed with primary antibody specifically against KCNQ2 (Cell Signaling Technology, #14752), RPH3A (Proteintech, #11396-1-AP), SV2A (Abcam, ab254351) at 4 °C overnight.

Techniques: Gene Expression, Control, Expressing

Journal: Scientific Reports

Article Title: Multiple Transcriptomic Analyses Explore Potential Synaptic Biomarker Rabphilin-3A for Alzheimer's Disease

doi: 10.1038/s41598-024-66693-8

Figure Lengend Snippet: Functional pattern similarity among three brain tissue datasets. ( A – C ) Lollipop plots illustrating shared downregulated pathways, with at least one of KCNQ2, RPH3A, SV2A , and TMEM59L , in the entorhinal cortex, superior frontal gyrus, and hippocampus, respectively.

Article Snippet: After blocking and washing, the membranes were probed with primary antibody specifically against KCNQ2 (Cell Signaling Technology, #14752), RPH3A (Proteintech, #11396-1-AP), SV2A (Abcam, ab254351) at 4 °C overnight.

Techniques: Functional Assay

Journal: Scientific Reports

Article Title: Multiple Transcriptomic Analyses Explore Potential Synaptic Biomarker Rabphilin-3A for Alzheimer's Disease

doi: 10.1038/s41598-024-66693-8

Figure Lengend Snippet: Expression level of RPH3A, KCNQ2, and SV2A in the hippocampus of wildtype (WT) and AD (3xTg-AD) mice. ( A ) Western blotting of 3 candidate proteins in mouse hippocampus brain regions, three mice in each group were examined. The samples derive from the same experiment and that gels/blots were processed in parallel. Original blots are presented in Supplementary Figs. – . ( B ) Bar graph demonstrates the statistical results of the Western blots in WT and 3xTg-AD groups. **p < 0.01.

Article Snippet: After blocking and washing, the membranes were probed with primary antibody specifically against KCNQ2 (Cell Signaling Technology, #14752), RPH3A (Proteintech, #11396-1-AP), SV2A (Abcam, ab254351) at 4 °C overnight.

Techniques: Expressing, Western Blot